Description
The 33 monoclonal antibody specifically recognizes Tunica interna endothelial cell kinase-2 (Tie2) which is also known as CD202b or Angiopoietin-1 receptor. Tie2 (CD202b) is a single-pass type I membrane protein that is encoded by TEK (TEK receptor tyrosine kinase) which belongs to the receptor tyrosine kinase (RTK) family. The extracellular region of Tie2 (CD202b) contains an N-terminal IgC2-like domain followed by three EGF-like domains, an additional IgC2-like domain and three fibronectin type III domains. The intracellular region contains a protein kinase domain. TIE receptors, designated Tie1 and Tie2 (Tek) have a tissue distribution which is limited primarily to cells of endothelial origin. Binding of angiopoietin 1 to Tie2 (CD202b) leads to downstream receptor-mediated signaling that is involved in angiopoiesis and survival of endothelial cells. Tie2 has been shown to be required for the normal development of vascular structures during embryogenesis. It has also been demonstrated to play a role in normal and pathological angiogenesis in adults. Mutations in the TEK gene cause multiple cutaneous and mucosal venous malformations (VMCM). Tie2 has a predicted molecular weight of 126 kD, but may be observable at ~130-165 kDa. This antibody has been reported to recognize human and mouse Tie2 but not Tie1.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
