Description
The 1A5 monoclonal antibody specifically recognizes B7 homolog 6 (B7-H6). B7-H6 is a ~51 kDa type I transmembrane glycoprotein that is encoded by NCR3LG1 (natural killer cell cytotoxicity receptor 3 ligand 1) which belongs to the B7 family of immunoreceptors. B7-H6 contains one IgV-like and one IgC1-like domain in its extracellular region followed by a transmembrane region and an intracytoplasmic region with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an SH2- and SH3-binding motifs. Although B7-H6 is not expressed by normal cells from healthy individuals, it is variably expressed on stressed cells including tumor cells and tumor cell lines, and can be moderately expressed on proinflammatory monocytes and neutrophils. Binding of B7-H6 to CD337 (NKp30/NCR3) induces NK cell activation, cytotoxicity, and cytokine secretion which suggests it may play an important role in immunosurveillance against certain tumors and infectious diseases.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
