Description
The 12.14 monoclonal antibody specifically recognizes Nur77, a zinc-finger transcription factor also known as Nerve growth factor IB (NGFI-B), Testicular receptor 3 (TR3), or Nuclear protein N10. Nur77 is encoded by Nr4a1 (Nuclear receptor subfamily 4 group A member 1), an immediate-early response gene that belongs to the nuclear receptor superfamily. This inducible orphan nuclear receptor is comprised of an N-terminal transactivation domain, followed by a central DNA-binding domain and a putative C-terminal ligand-binding domain for which no ligand has been identified. In electrophoretic analyses, Nur77 migrates as diffuse protein bands between 67 and 88 kDa depending on its level of phosphorylation or other post-translational modifications. Nr4a1 expression can be rapidly induced by diverse stimuli in cells from primary and secondary lymphoid tissues and other tissues including the brain, muscle, ovary, and testis. Nur77 expression is rapidly upregulated by antigen-stimulated mouse thymocytes and may promote activation- induced apoptosis during negative selection. Ag receptor-mediated signaling by B cells, T cells, or T cell hybridomas can also lead to rapid upregulated Nur77 expression which may regulate cellular proliferation and survival. In response to certain growth factors, cytokines, inflammatory mediators, or stress-inducing stimuli, other cell types, including myeloid or stromal cell types, can upregulate Nur77 expression which affects their growth, differentiation, proliferation or survival. Clone 12.14 recognizes mouse Nur77 and reportedly crossreacts with human Nur77.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
