Description
The 34-1-2S monoclonal antibody specifically recognizes the mouse histocompatibility 2 (H-2) alloantigens, H-2Kd and H-2Dd, that are variably expressed on most nucleated cells. These MHC class I antigens are heterodimers comprised of a polymorphic alpha heavy chains (~44 kDa type I transmembrane glycoprotein encoded by the H-2 gene complex) that are noncovalently associated with invariant β2-microglobulin (~11 kDa beta light chain encoded by B2m). H-2Kd and H-2Dd are involved in the positive and negative selection of CD8+ T cells in the thymus as well as MHC-restricted antigen presentation to CD8+ αβ T cells in the periphery. H-2Kd and H-2Dd molecules can also serve a ligands for activating or inhibitory receptors, such as, those encoded by the Ly49 gene family which are variably expressed on subsets of natural killer (NK) cells and T cells. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
8. Alexa Fluor™ is a trademark of Life Technologies Corporation.
9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
