Description
The MH22B2 monoclonal antibody specifically recognizes human Interleukin-22 (IL-22), which is encoded by the IL22 gene. Cross-reactivity of MH22B2 mAb to mouse IL-22 (which shares 79% amino acid identity with human IL-22) has been observed by ELISA and by flow cytometry of HEK293 cells transfected with mouse IL-22 and TH17-differentiated CD4-positive T cells from C57BL/6 mice. IL-22 (with or without other cytokines) is secreted by many T-cell and innate-lymphoid-cell populations. Evidence that IL-22 plays a key role in mucosal immunity includes the restricted expression of the alpha subunit of the heterodimeric IL-22 receptor, called IL-22R1, on epithelial cells and cells of epithelial origin. At epithelial surfaces, IL-22 elicits antimicrobial defenses and maintains epithelial integrity. Alternatively, uncontrolled IL-22 production can result in certain inflammatory disorders. Regulation of IL-22 expression is complex, involving other cytokines (eg, IL-6, IL-23, and TGF-β) and many transcription factors, (eg, AHR, c-Maf, STAT3, RORɤT, BATF, and others).
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. APC-Cy7 is a tandem fluorochrome composed of Allophycocyanin (APC), which is excited by laser lines between 595 and 647 nm and serves as an energy donor, coupled to the cyanine dye Cy7™, which acts as an energy acceptor and fluoresces at 780 nm. BD Biosciences Pharmingen has maximized the fluorochrome energy transfer in APC-Cy7, thus maximizing its fluorescence emission intensity, minimizing residual emission from APC, and minimizing required electronic compensation in multilaser-laser flow cytometry systems. Note: Although every effort is made to minimize the lot-to-lot variation in residual emission from APC, it is strongly recommended that every lot be tested for differences in the amount of compensation required and that individual compensation controls are run for each APC-Cy7 conjugate.
5. APC-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.
6. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
9. Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036).
10. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
12. For U.S. patents that may apply, see bd.com/patents.
