Description
The H32-411 monoclonal antibody specifically recognizes B-cell activating factor (BAFF) which is also known as B-lymphocyte stimulator protein (BLyS), TNF and ApoL-related leukocyte expressed ligand 1 (TALL1), Dendritic cell-derived TNF-like molecule (DTL) and CD257. BAFF is a type II transmembrane glycoprotein that is encoded by TNFSF13B (TNF superfamily member 13b). BAFF can be expressed on B cells, macrophages, and dendritic cells as well as on tumor cells. Membrane and soluble forms of BAFF have been described. In its soluble form, BAFF can serve as a potent B cell growth factor in costimulation assays, suggesting its biological role in monocyte-driven B cell activation. BAFF has also been linked to B cell hyperplasia and autoimmunity.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
5. An isotype control should be used at the same concentration as the antibody of interest.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
