Description
The 1A5 (1A5/SIRL-1) monoclonal antibody specifically recognizes human V-set and transmembrane domain-containing protein 1 (VSTM1) which is also known as Signal Inhibitory Receptor on Leucocytes-1 (SIRL-1). VSTM1 (SIRL-1) is a single-pass type I transmembrane glycoprotein that contains an IgV-like domain in its extracellular region which is followed by a transmembrane segment and an intracellular region with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). This molecule is encoded by VSTM1 (V-set and transmembrane domain containing 1) which belongs to the transmembrane receptor Ig superfamily of immune inhibitory receptors. VSTM1 (SIRL-1) is variably expressed on myeloid cells including neutrophils, eosinophils, and monocytes. SIRL-1 recognizes phenol-soluble modulins of Staphylococcus as well as danger- or damage-associated molecular patterns (DAMPs) including S100 proteins and the antimicrobial peptide, LL-37. VSTM1 (SIRL-1) regulates innate effector functions including Fc Receptor-induced production of reactive oxygen species (ROS) and formation of neutrophil extracellular traps (NET) released by human neutrophils in response to certain bacterial and inflammatory danger signals. Although NETs serve to trap and kill extracellular microbes, VSTM1 (SIRL-1)-mediated regulation of NETs expression helps avoid excessive tissue damage caused by inflammation and potential induction or exacerbation of autoimmune disease. VSTM1 (SIRL-1) function does not impair neutrophil phagocytosis and killing of intracellular bacteria. VSTM1 (SIRL-1) expression is downregulated in response to microbial stimuli which allows microbial killing and clearance of pathogens.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. An isotype control should be used at the same concentration as the antibody of interest.
7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
11. For U.S. patents that may apply, see bd.com/patents.
