Description
The NP57 monoclonal antibody specifically recognizes human Neutrophil Elastase (NE) which is also known as Human leukocyte elastase (HLE), Granulocyte-derived Elastase (GE), or PMN Elastase (PMN-E). Neutrophil Elastase is a major serine proteinase encoded by ELANE (Elastase, neutrophil expressed) that belongs to the elastase subfamily within the peptidase S1 family. Neutrophil Elastase is predominantly present in the azurophilic granules (primary granules) of neutrophils and monocytes. When released outside of these effector cells, it can act to hydrolyze a variety of extracellular matrix substrates including elastin, fibronectin, collagen, and proteoglycans. Elastase activity in normally controlled by serine proteinase inhibitors. Neutrophil Elastase can regulate the functions of NK cells, monocytes, and granulocytes, eg, by inhibiting C5a-dependent enzyme release and chemotaxis by neutrophils. It is also capable of killing bacteria such as E. coli and degrading virulence factors from bacteria such as Yersinia, Shigella, and Salmonella. Dysregulated expression or defects in this enzyme are associated with some inflammatory and degenerative diseases including rheumatoid arthritis, emphysema, chronic obstructive pulmonary disease, and cystic fibrosis as well as neutropenias.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
10. For U.S. patents that may apply, see bd.com/patents.
