BD, 570008, Alexa Fluor™ 488 Mouse Anti-Human CCR10 1B5

Name: Alexa Fluor™ 488 Mouse Anti-Human CCR10 1B5
Brand: BD
SKU: 570008

0.1 mg

Catalog Number: 570008

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Category

Cell Cycle Reagents

Pack Size108 units / pack

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The 1B5 monoclonal antibody specifically binds to CCR10 (CC-chemokine receptor 10). CCR10 is a seven transmembrane domain G-protein-coupled receptor that is also known as GPR-2 (G-protein coupled receptor 2). CCR10 is expressed by cells that mediate epithelial immunity including a small subset of T memory cells, IgA-secreting B cells, dermal endothelial cells and fibroblasts. It is also expressed on EBV-immortalized B cells. Chemokines CCL27 and CCL28 are the ligands for CCR10. CCR10 reportedly not only functions in regulating the homing and function of cells that mediate epithelial immunity. It may also regulate the epithelial localization, survival, and proliferation of tumor cells and facilitate their evasion from immune surveillance.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. An isotype control should be used at the same concentration as the antibody of interest.
4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
10. For U.S. patents that may apply, see bd.com/patents.

Specifications

General

Source	Human IL-1α, amino acids Ser113-Ala271 (Accession# NM_000575) was expressed in E. coli.
Molecular Mass	The 159 amino acid recombinant protein has a predicted molecular mass of 18,047 Da. The DTT-reduced and the non-reduced protein migrate at approximately 18kDa by SDS-PAGE. The N-terminal amino acid is Serine.
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level	Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration	10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

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