Description
The 1D6 monoclonal antibody specifically binds to CD81 which is also known as, Target of the antiproliferative antibody 1 (TAPA1 or TAPA-1), Tetraspanin-28 (Tspan-28 or TSPAN28), or Common variable immunodeficiency 6 (CVID6). This ~26 kDa surface protein is encoded by CD81 that belongs to the tetraspanin or transmembrane 4 (TM4SF) superfamily. CD81 is composed of four transmembrane and two extracellular domains with intracellular N- and C-termini. CD81 has a broad cellular distribution. It is variably expressed on thymocytes, T lymphocytes, B cells, NK cells, eosinophils, germinal center follicular dendritic cells, monocytes, endothelial cells, epithelial cells, fibroblasts, and at low levels on neutrophils. CD81 is involved in signal transduction and the regulation of cellular growth and adhesion. It serves as a component of a multimolecular complex comprised of CD19, CD21, and CD225 that regulates the activation and growth of B cells. Abnormal CD81 expression is implicated in the growth regulation of some lymphomas and in CVID6 immunodeficiency which is characterized by low antibody production. CD81 can also serve as a receptor for hepatitis C virus (HCV). The 1D6 antibody can reportedly induce homotypic adhesion amongst hematolymphoid cells, inhibit the proliferation of some B-cell lines, and costimulate anti-CD3 antibody activated T cells.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. An isotype control should be used at the same concentration as the antibody of interest.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
9. For U.S. patents that may apply, see bd.com/patents.
