Description
The P6-1 monoclonal antibody specifically recognizes the extracellular region of Human Epidermal Growth Factor Receptor 4 (HER4). HER4 is encoded by ERBB4 (erb-b2 receptor tyrosine kinase 4). HER4 (ErbB4) belongs to the epidermal growth factor receptor subfamily within the ERBB family of Receptor Tyrosine Kinases (RTK) that includes HER1 (EGFR), HER2 (ErbB2), and HER3 (ErbB3). HER4 (ErbB4) is an ~180 kDa type I transmembrane glycoprotein that contains an extracellular ligand-binding region, a transmembrane sequence, and a cytoplasmic tail with the active tyrosine kinase domain. This RTK functions in a variety of normal cellular processes including proliferation, differentiation, migration, survival, and apoptosis that are often dysregulated in cancer cells. HER4 (ErbB4) is prominently involved in regulating the development and maintenance of the heart, central nervous system, and mammary gland. Upon binding ligands, including neuregulins and EGF family members, HER4 (ErbB4) can homodimerize or heterodimerize with other family members and transmit intracellular signals involved in initiating and regulating cellular responses. High expression of ERBB4 occurs in brain, heart, kidney, and heart as well as skeletal muscle, bone marrow mesenchymal stem cells and breast tissue. Overexpression of and mutations in the ERBB4 gene have been associated with some leukemias, neuroblastomas, and carcinomas. The P6-1 antibody can reportedly suppress Neuregulin-1 (NRG-1)-dependent signaling and colony formation responses mediated through HER4 (ErbB4) expressed on human breast cancer cell lines.
Bead-based compensation or unmixing controls, such as BD® CompBeads or BD™ SpectraComp™, can be used as surrogates to assess fluorescence spillover when bound to fluorochrome-conjugated antibodies. Although these beads have spectral properties similar to cells, variations in spectral emission may occur, resulting in differing spillover values compared to biological controls. Therefore, it is considered best practice to compare the spillover obtained from cells and bead-based controls when using BD® CompBeads or BD™ SpectraComp™ for the first time, to ensure they are appropriate for the intended application.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. An isotype control should be used at the same concentration as the antibody of interest.
7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
8. For U.S. patents that may apply, see bd.com/patents.
