Description
The B3506B4 monoclonal antibody specifically recognizes the Fc region of the human Immunoglobulin A (IgA) subclass known as IgA1. IgA1 is comprised of two identical heavy chains encoded by IGHA1, and two light chains, either Igκ or Igλ, that are linked together by disulfide bonds. The B3506B4 antibody does not crossreact with other human immunoglobulin heavy chain (IgH) subclasses, including the IgA2 subclass. IgA1 is expressed by plasmablasts, plasma cells, and memory B cells as well as by some myeloma or plasmacytoma cells. IgA1 is expressed in either a transmembrane form that serves as an antigen-specific B cell surface receptor or in a soluble secreted form. Monomeric and dimeric forms of IgA1 are found in the serum although the monomeric form predominates. Multimeric forms of IgA1 connected by a J-chain and secretory component are also found in bodily secretions. Secretory IgA is transported across epithelial cells and secreted into the lumen of mucosal tissues including the gastrointestinal and respiratory tracts and is found in human breast milk. IgA1 functions in the agglutination of microbes and can block their adherence to or infection of human cells. It can also bind to CD89 (FcαRI) expressed on neutrophils, monocytes, macrophages, and eosinophils to trigger a variety of immune responses including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and the release of inflammatory cytokines and mediators such as in the response to microbes.
Bead-based compensation or unmixing controls, such as BD® CompBeads or BD™ SpectraComp™, can be used as surrogates to assess fluorescence spillover when bound to fluorochrome-conjugated antibodies. Although these beads have spectral properties similar to cells, variations in spectral emission may occur, resulting in differing spillover values compared to biological controls. Therefore, it is considered best practice to compare the spillover obtained from cells and bead-based controls when using BD® CompBeads or BD™ SpectraComp™ for the first time, to ensure they are appropriate for the intended application.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. An isotype control should be used at the same concentration as the antibody of interest.
8. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
11. For U.S. patents that may apply, see bd.com/patents.
