Description
The CC02-30 monoclonal antibody specifically recognizes inducible nitric oxide synthase (iNOS) which is also known as NOS type II (Nos-2 or NOS-II) or Macrophage NOS (MAC-NOS). This enzyme is encoded by Nos2 (nitric oxide synthase 2, inducible). The iNOS homodimer catalyzes the synthesis of nitric oxide (NO) in macrophages and other cell types that are induced to express iNOS in response to certain cytokines (eg, IFN-γ, TNF-α, and IL-1) and microbial products (eg, lipopolysaccharides, proteins, nucleic acids). iNOS acts through a multistep process to catalyze the generation of nitric oxide from L-arginine using molecular oxygen and NADPH as an electron donor. The NO free radical is highly reactive with other molecules such as proteins and nucleic acids of microbial origin and plays an important role in immunity by killing microbial pathogens to help counter infections. iNOS belongs to the NOS family that includes two other different nitric oxide synthases (isozymes): i) Neuronal NOS (nNOS encoded by Nos1) expressed in neurons and involved in neurotransmission as well as ii) Endothelial NOS (eNOS encoded by Nos3) expressed by endothelial cells and involved in regulating blood flow and vascular tone.
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
6. An isotype control should be used at the same concentration as the antibody of interest.
7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
10. For U.S. patents that may apply, see bd.com/patents.
