Description
The migration of T cells through lymph nodes and their interactions with antigen presenting cells (APCs) involve adhesion molecules on the surfaces of the T cell and the cell with which it interacts. These molecules include the selectins, the integrins, members of the immunoglobulin superfamily, and some mucin-like proteins. The integrins mediate adhesion between cells and between cells and the extracellular matrix. LFA-1 (lymphocyte function-associated antigen 1), a member of the ß2 integrin family, is expressed on T cells, neutrophils, and macrophages and is thought to be the most important adhesion molecule for lymphocyte activation. It interacts with members of the immunoglobulin superfamily, the intercellular adhesion molecules: ICAM-1, -2, and -3. ICAM-1 and ICAM-2 are expressed on endodthelium and APCs, while ICAM-3 is expressed only on leukocytes. ICAM-1 is most homologous to neural cell adhesion molecule and myelin-associated glycoprotein. It is a 55 kDa glycoprotein that is heavily glycosylated to form 90 kDa to 115 kDa proteins. Its interactions with LFA-1 are necessary for CTL and NK mediated killing, T and B cell responses, and immune cell extravasation.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
