BD, 612656, Purified Mouse Anti-Actin Ab-5 C4/actin

Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments results from interactions between actin and actin-binding proteins. Actin is a 42-kDa protein that is known as G-actin in its monomeric form. Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin. F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks. The latter are highly concentrated beneath the plasma membrane and form the actin cortex. Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins. These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures. Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.