Description
The SAM.2.rMAb is a recombinant monoclonal antibody that recognizes TCR Cβ2 expressed by a large proportion of CD4+ and CD8+ T cells. Thymocytes and mature peripheral T cells predominantly express a heterodimeric T cell receptor (TCR αβ) for antigen which is comprised of disulfide-liked transmembrane α and β chain subunits. The constant region of the TCR α subunit is encoded by TRAC, whereas the TCR β subunit is encoded by either of two highly homologous constant region genes, TCRB1 for TCR Cβ1 or TCRB2 for TCR Cβ2. The JOVI.1 antibody alternatively recognizes TCR Cβ1 expressed by the other TCR αβ+ T cells. These antibodies are effectively used together in multicolor staining and flow cytometric analyses to identify and characterize the natures of either TCR Cβ1+ or TCR Cβ2+ T cells in heterogeneous cell populations.
The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry. Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.
1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
3. For U.S. patents that may apply, see bd.com/patents.
4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
5. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
6. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
8. An isotype control should be used at the same concentration as the antibody of interest.
9. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
10. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
