Description
The RMV-7 monoclonal antibody specifically binds to CD51, the 140 kDa integrin αV chain. Heterodimers of CD51 with several integrin β chains function as receptors for extracellular matrix proteins. CD51/CD61 (αVβ3 integrin, vitronectin receptor) mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, von Willebrand factor, and CD31 (PECAM-1). CD51 is expressed on activated T lymphocytes, polymorphonuclear granulocytes, blastocysts, and osteoclasts. CD51 is reportedly not detectable on mouse platelets using either H9.2B8 or RMV-7 antibody clones. CD51 also forms heterodimers with CD29 (integrin β1), integrins β5, β6, and β8 chains. αV integrins have diverse functions in development and homeostasis. The RMV-7 antibody reportedly blocks LAK-cell binding to vitronectin, fibronectin, fibrinogen, and CD31. Furthermore, the RMV-7 antibody reportedly inhibits LAK-cell cytoxicity against certain target cells by interfering with the binding of LAK cells to their target cells.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
10. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
