BD, 742713, BV711 Mouse Anti-Human Alkaline Phosphatase B4-78/Alkaline Phosphatase Bone and Liver

Name: BV711 Mouse Anti-Human Alkaline Phosphatase B4-78/Alkaline Phosphatase Bone and Liver
Brand: BD
SKU: 742713

50 µg

Catalog Number: 742713

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Category

Fluorescent-Conjugated Antibodies for FACS

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The B4-78 monoclonal antibody reacts with the tissue-nonspecific isozyme of alkaline phosphatase. Alkaline phosphatases are membrane-bound glycoproteins. Four isozymes of alkaline phosphatase exist in humans: placental, placental-like, intestinal, and liver/bone/kidney. Liver/bone/kidney alkaline phosphatase is also known as tissue-nonspecific alkaline phosphatase (TNAP). Human embryonic stem cells and embryonic carcinoma cells express high levels of tissue-nonspecific alkaline phosphatase that decrease upon differentiation. Genetic and biochemical studies suggest that TNAP plays a role in skeletal mineralization.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
10. Cy is a trademark of GE Healthcare.
11. Alexa Fluor® is a registered trademark of Life Technologies Corporation.

Specifications

General

Brand	BD OptiBuild™
Alternative Name	Bone/Kidney/Liver Alkaline Phosphatase, TNAP, TNSALP, AP-TNAP, ALPL
Reactivity	Human (Tested in Development)
Isotype	Mouse BALB/c IgG1, κ
Immunogen	Human Bone Alkaline Phosphatase
Application	Flow cytometry (Qualified)
Concentration	0.2 mg/ml
RRID	AB_2740992
Storage Buffer	Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status	RUO

FAQs

Question: What is the antibody concentration?

Answer:The BV711 conjugate is supplied at 0.2 mg/mL.

Question: When should Brilliant Stain Buffer be used?

Answer:Use it when two or more BD Horizon Brilliant dyes are present in the same experiment to reduce dye-interaction artifacts.

Question: How should the reagent be stored?

Answer:Store undiluted at 4 °C, protect from prolonged light exposure, and do not freeze.

Application Note

BV711 Mouse Anti-Human Alkaline Phosphatase, Clone B4-78 is used for flow-cytometric detection of human tissue-nonspecific alkaline phosphatase, including the bone, kidney, and liver form associated with ALPL. The monoclonal antibody has a mouse BALB/c IgG1, κ isotype and was generated against human bone alkaline phosphatase. Human reactivity was tested during development, and the flow-cytometry application is qualified. The antibody is affinity purified from tissue-culture supernatant or ascites and directly conjugated with BD Horizon BV711 under conditions that minimize unconjugated dye and antibody. The finished reagent has a concentration of 0.2 mg/mL in an aqueous buffered solution containing no more than 0.09% sodium azide. Determine the working concentration for the sample type and panel, and use an isotype control at the same concentration when background control is needed. When two or more BD Horizon Brilliant dyes are included in one experiment, add BD Horizon Brilliant Stain Buffer to reduce dye interactions that can create staining artifacts and complicate interpretation. Store the antibody undiluted at 4 °C, protect it from prolonged light exposure, and do not freeze it. Direct BV711 conjugation supplies a violet-laser-compatible signal without a fluorescent secondary antibody, while the defined concentration supports controlled titration during panel optimization. This combination suits multicolor research panels that need a human alkaline-phosphatase marker together with other surface or intracellular targets. The reagent is for research use only and not for diagnostic or therapeutic procedures.

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