BD, 745079, BV510 Mouse Anti-Human CD137 4B4-1

Name: BV510 Mouse Anti-Human CD137 4B4-1
Brand: BD
SKU: 745079

50 µg

Catalog Number: 745079

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Category

Fluorescent-Conjugated Antibodies for FACS

TagsReagents Flow Cytometry Reagents research-reagents

CollectionsBD Flow Cytometry Reagents for Multicolor Panels BD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels

Description

The 4B4-1 monoclonal antibody specifically binds to CD137 which is also known as 4-1BB, and ILA (induced by lymphocyte activation). CD137 is a type I transmembrane glycoprotein that belongs to the TNF/NGF receptor family. It is encoded by TNFRSF9 (tumor necrosis factor receptor superfamily, member 9). CD137 is expressed on activated T cells, B cells, monocytes, and follicular dendritic cells. CD137 plays roles in the costimulation, differentiation and survival of T cells and B cells.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.

Specifications

General

Brand	BD OptiBuild™
Alternative Name	4-1BB, ILA, TNFRSF9
Reactivity	Human (Tested in Development)
Isotype	Mouse BALB/c IgG1, κ
Immunogen	Recombinant Human 4-1BB Fusion Protein
Application	Flow cytometry (Qualified)
Concentration	0.2 mg/ml
Workshop Number	VI C-7
RRID	AB_2742694
Storage Buffer	Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status	RUO

FAQs

Question: What is the supplied concentration?

Answer:The BV510 conjugate is supplied at 0.2 mg/mL.

Question: Is a fixed volume per test provided?

Answer:No fixed per-test volume is provided; titrate the reagent for the individual application.

Question: How should the antibody be stored?

Answer:Store it undiluted at 4 °C, protect it from prolonged light exposure, and do not freeze it.

Application Note

BV510 Mouse Anti-Human CD137, Clone 4B4-1 is used for flow-cytometric detection of human CD137, also known as 4-1BB, ILA, or TNFRSF9. The monoclonal antibody has a mouse BALB/c IgG1, κ isotype and was generated against a recombinant human 4-1BB fusion protein. Human reactivity was tested during development, and flow-cytometry use is qualified. Affinity purification isolates the antibody from tissue-culture supernatant or ascites, followed by direct conjugation with BD Horizon BV510 under conditions that minimize free dye and unconjugated antibody. The reagent is supplied at 0.2 mg/mL in an aqueous buffered solution containing no more than 0.09% sodium azide. Titrate the antibody for the sample type and staining panel, and use an isotype control at the same concentration when the design requires background assessment. When two or more BD Horizon Brilliant dyes are present in the same experiment, include BD Horizon Brilliant Stain Buffer to minimize fluorescent-dye interactions that may create staining artifacts. Store the antibody undiluted at 4 °C, protect it from prolonged light exposure, and do not freeze it. Direct BV510 attachment provides a ready fluorescent primary antibody for violet-laser multicolor panels, and the defined concentration supports controlled titration rather than a fixed test volume. Clone identity, isotype, immunogen, human reactivity, and qualified application support experimental planning and reporting. The antibody is intended only for research flow cytometry, not diagnostic or therapeutic procedures.

Ordering

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