Description
The HMFG2 (Milk Fat Globule 2) monoclonal antibody, formerly known as clone 3.14.A3, specifically binds to Mucin-1 (MUC1) which is also known as CD227, Episialin, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, H23 antigen, Peanut reactive urinary protein (PUM), Polymorphic epithelial mucin (PEM), or Epithelial membrane antigen (EMA). MUC1 (CD227) is a heterodimeric cell surface glycoprotein that belongs to the epithelial mucin family. As a result of proteolytic cleavage, MUC1 (CD227) is comprised of a large, extracellular N-terminal alpha subunit that contains a domain of 20 amino-acid tandem repeats and functions in cellular adhesion. The smaller transmembrane C-terminal beta subunit contains a cytoplasmic domain that is involved in cell signaling. MUC1 (CD227) is variably expressed on the surfaces of normal and malignant glandular and ductal epithelial cells, myeloma cells, and some hematopoietic cell lineages including subsets of T cells, B cells, monocytes and dendritic cells. Soluble forms of MUC1 (CD227) may arise by shedding from the cell surface or by secretion of forms derived from alternative RNA splicing. MUC1 (CD227) plays roles in the provision of protective barrier function, the regulation of cellular adhesion and migration, and the transduction of multiple signal pathways. The HMFG2 antibody recognizes epitopes within the tandem repeat domain of MUC1 (CD227).
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BB700 under optimal conditions that minimize unconjugated dye and antibody.
1. This antibody was developed for use in flow cytometry.
2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
3. Researchers should determine the optimal concentration of this reagent for their individual applications.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
9. BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.
10. Cy is a trademark of GE Healthcare.
