Bio-Rad, 12003233, Nuvia™ IMAC Resin, 5 L

Name: Nuvia™ IMAC Resin, 5 L
Brand: Bio-Rad
SKU: 12003233

Pkg of 1, 5 L, ready-to-use Ni-charged nitrilotriacetic acid (NTA) support for immobilized metal affinity chromatography, Nuvia™ IMAC Resin, 5 L

Catalog Number: 12003233

Pricing

Particle size	38–53 µm
Ligand density	≥ 18 µmol/ml
Dynamic binding capacity*	> 40 mg/ml at 300 cm/hr *10% breakthrough capacity determined with 1.2 mg/ml of a 40 kD histidine-tagged protein in 50 mM sodium phosphate, 5 mM imidazole, 300 mM NaCl (pH 7.5).
Compression factor	1.20–1.25
Recommended linear flow rate range	50–300 cm/hr
Pressure vs. flow performance	Under 2 bar at flow rate of 300 cm/hr in DI water (20 x 20 cm packed bed, 1.2 compression factor)
pH stability	2–14
Shipping solution	2% benzyl alcohol or 20% ethanol
Regeneration	50 mM EDTA, pH 8.0 (Stripping) 1N NaOH (CIP/SIP) 0.1 M Ni2SO4 (Recharging)
Cleaning-in-place (CIP) solutions	1N NaOH
Sanitization	1N NaOH
Storage condition	20% Ethanol
Chemical compatibility	Reducing agents (β-ME, TCEP, DTT) Denaturing agents (GnHCl) Detergents (octylphenol ethoxylate, NP-40, CHAPS, CHAPSO) Additives (glycerol)
Chemical compatibility note	No recharge required. Refer to the instruction manual for a complete list.
Chemical stability	48 hr with 1M NaOH, 1 week with 0.01M HCl

Ordering

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