Description
Go further with your Out of the Blue CRISPR Kit results!
With the Out of the Blue Genotyping Extension Kit, students use multiplex PCR and agarose gel electrophoresis to confirm the edit they made to the lacZ gene with the Out of the Blue CRISPR Kit (sold separately). A key step in CRISPR and gene editing research workflows, this genotyping activity offers students an opportunity to engage in the scientific process of compiling evidence from multiple sources to explain a phenomenon.
See the Out of the Blue CRISPR and Genotyping Extension Kits product page for more information.
The Out of the Blue Genotyping Extension requires use of the Out of the Blue CRISPR Kit (12012608EDU) and electrophoresis reagents (agarose, TAE buffer, DNA stain), sold separately.
Contents
Each kit contains the following materials for 8 student workstations (2–4 students per workstation).
Items to store at -20°C (components of the Out of the Blue Genotyping Extension Refill Pack, 12012708EDU):
• 2x PCR Master Mix
• Primer Mix
• PCR Positive Control DNA
• Molecular Weight Ruler
• Orange G Loading Dye
Items to store at room temperature:
• InstaGene Matrix
• 1.5 ml Conical tubes with O-ring screw caps, 50
• 1.5 ml Microcentrifuge tubes, 60
• 0.2 ml PCR tubes, 100
• Instructor Answer Guide
Out of the Blue CRISPR and Genotyping Kit Options
Out of the Blue CRISPR and Genotyping Extension Kits are available in a selection of configurations and bundles to save you money and allow you to choose the best DNA staining methods for your needs. Refill Packs are also available (see the Refills tab).
Related Equipment
• T100 Thermal Cycler
• Mini-Sub Cell GT Systems
• PowerPac Basic Power Supply
• Micropipets
Supporting Documents
We've gone green! Download the student and instructor manuals.
CRISPR Teaching Resources
CRISPR-Cas Gene Editing Teaching Resources
