Description
Use the ddPLEX EGFR/KRAS/BRAF Mutation Detection Kit * to detect 37 key non-small cell lung cancer (NSCLC) mutations. This kit provides all necessary reagents for detecting these variants and their wild-type counterparts using cell-free DNA (cfDNA) in plasma and/or DNA from tumor tissue samples, enabling calculation of variant allele frequencies.
When paired with Bio-Rad’s QX600 TM Droplet Digital TM PCR (ddPCR TM ) System * and using the recommended controls and guidelines, labs can process up to 42 unknown samples per plate and complete sample loading to data generation in under 8 hours.
* For Research Use Only. Not for use in diagnostic procedures.
Features and Benefits
• High sensitivity: detect established NSCLC mutations at ≤0.1% VAF ** in a single well
• Accelerated turnaround: achieve same-day results with a streamlined workflow
• Versatile sample input: compatible with plasma or formalin-fixed paraffin embedded (FFPE) samples for greater flexibility
• Built-in quality assurance: includes an internal control; positive control available separately
• Enhanced detection power: atented wild-type suppression chemistry boosts sensitivity
• Reliable performance: powered by ddPCR technology using the QX600 ddPCR System and QX Manager Software (SE v2.1 or higher) for exceptional reproducibility and specificity
** Variant Allele Frequency
Product Contents
This kit includes the following components for 120 reactions.
• 12023484 - ddPLEX EGFR/KRAS/BRAF Mutation Detection Assays - Qty 1 ddPLEX EGFR/KRAS/BRAF Total Quantification Assay ddPLEX EGFR/KRAS/BRAF Mutant Assay ddPLEX Enhancer ddPLEX Internal Control
Additional reagents required but not included:
• 12023522 - ddPLEX EGFR/KRAS/BRAF Positive Control – Qty 1
• 12005909 - ddPCR Multiplex Supermix – Qty 1
• 12012171 - Dithiothreitol (DTT) – Qty 1
Configuration Options
• The ddPLEX EGFR/KRAS/BRAF Mutation Detection Kit should be used with the QX600 ddPCR System and either the Manual Droplet Generator or Automated Droplet Generator.
• Catalog # 17010491 can alternatively be used to order all four of the required component kits (assays, positive control, Supermix, and DTT).
Key Droplet Generation & Reader Equipment, Consumables and Reagents
Reagents & Consumables for Automated Droplet Generation Only
Reagents & Consumables for Manual Droplet Generation Only
Related Products
• BRAF V600 ddPCR Multiplex Mutation Screening Kit
• KRAS G12/G13 ddPCR Multiplex Mutation Screening Kit
• EGFR Exon19 Deletions ddPCR Multiplex Mutation Kit
• ddPLEX ESR1 Mutation Detection Kit
• ddPCR™ Expert Design Assay: EML4-ALK, Human, Homo sapiens
• ddPCR™ Expert Design Assay: ROS1 mRNA Fusions, Human, Homo sapiens
• ddPCR™ Expert Design Assay: RET mRNA Fusions, Human, Homo sapiens
Related Categories
• ddPCR Multiplex Mutation Screening Kits
For Research Use Only. Not for use in diagnostic procedures.
