Bio-Rad, 1351201, CFDA-SE

The CFDA-SE (5[6]-carboxyfluorescein diacetate succinimidyl ester) cell proliferation assay is packaged in 100 μg vials. Simply reconstitute it with DMSO for use and avoid weighing and wasting reagents. CFDA-SE is a cell-permeable reagent that is useful in measuring and tracking cell divisions. Upon entering a live cell, the acetate groups of CFDA-SE are cleaved by intracellular esterase to create the fluorescent carboxyfluorescent carboxyfluorescein succinimidyl ester (CFSE) compound. CFSE reacts with free primary amines to create a stable, covalent bond and is retained in the cytosol of cells. As a cell divides, the fluorescence intensity of CFSE is successively halved with each division, allowing each cell generation to be distinguished.

Storage Information
Store at -20°C only
This product is photosensitive and should be protected from light