Send an RFQ for Bio-Rad 1610301, SDS (Sodium Dodecyl Sulfate). Iright supports global sourcing and international shipment for life science and laboratory products.
Answer:SDS denatures proteins and imparts a largely uniform negative charge to support molecular-size-based separation.
Answer:No. The 100 g presentation is powdered sodium dodecyl sulfate.
Answer:Yes. Residual SDS can interfere with enzymes, protein interactions and some analytical techniques; removal depends on the subsequent method.
Bio-Rad SDS (Sodium Dodecyl Sulfate) is an anionic detergent commonly used to denature proteins and impart a largely uniform negative charge for SDS-PAGE. The 100 g powdered reagent is used to prepare electrophoresis sample buffers, gel systems and other detergent-containing solutions. When combined with suitable sample preparation and electrophoresis conditions, SDS reduces differences caused by native protein charge and conformation so migration primarily reflects molecular size. It may also be used in selected membrane protein solubilization and cell-lysis formulations where an anionic surfactant is appropriate. Solution concentration is selected for the protocol and the required degree of denaturation. SDS can interfere with enzyme activity, binding assays and downstream analytical methods, so detergent removal may be necessary after sample preparation. This is dry detergent powder, not a premixed 10% or 20% SDS solution.
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