Description
Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development.
Applications and Uses
• Monitoring electrophoretic separation
• Monitoring blot transfer efficiency with easy orientation via multicolor standards
• Molecular weight sizing of proteins after blot development
• Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range
Packaging Options
More Information
Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3 , 0.01% bromophenol blue.
Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.
Related Products
• Precision Protein StrepTactin-HRP Conjugate, 300 µl ( 1610380 )
• Precision Protein StrepTactin-HRP Conjugate, 125 µl ( 1610381 )
• StrepTactin-AP Conjugate, 300 µl ( 1610382 )
More Precision Plus Protein Standards:
• Precision Plus Protein Unstained Standards
• Precision Plus Protein Prestained Standards
• IEF and 2-D Electrophoresis Protein Standards
Related Categories
• Protein Ladders and Standards
• Precast Protein Gels
• Protein Electrophoresis and Blotting
• Western Blotting
Supporting Documents
• Precision Plus Protein WesternC Standards Instruction Manual
• StrepTactin HRP-Conjugate and AP-Conjugate Instruction Manual
Online Resources
• Protein Electrophoresis
• Western Blotting
