Description
Choose economical, sensitive Flamingo Fluorescent Protein Gel Stain for the detection of denatured proteins in gels including first-dimension isoelectric focusing (IEF) gels. The dye is non-fluorescent in solution and becomes strongly fluorescent on binding to protein.
• Linear response over 3 orders of magnitude, with a lower limit of 0.25–0.5 ng
• No destaining required, and gels cannot be overstained
• Low background; readily distinguish spots and speckles
• Stain may be exposed to typical room light for up to 8 hours without affecting the fluorescence intensity of stained protein bands or spots
• Stained gels may be stored in the dark at 2–8°C for up to 6 months without significant loss of imaging sensitivity
Flamingo Fluorescent Protein Gel Stain can be visualized with laser-based gel imaging systems or UV detectors (Ex 512 nm, Em 535 nm, secondary Ex 271 nm; see Specifications tab for laser/emission filter compatibility). The staining procedure is a simple two-step protocol that can be completed in as little as 5 hours. The steps are not time-sensitive. Stained proteins are compatible with mass spectrometry and enzymatic digestion.
Packaging Options
Related Products
• High-Throughput Dodeca™ Gel Stainers
• PROTEAN ® i12™ IEF System
• 10% Tween 20 ( 1610781 )
• ReadyPrep™ Proteomics Grade Water ( 1632091 )
• Criterion™ Staining/Blotting Trays with lids ( 3459921 )
• Storage Box, small ( 1653430 )
• Storage Box, large ( 1653429 )
Related Categories
• Protein Gels
• Electrophoresis Chambers
• Gel Imaging Systems
• First-Dimension Isoelectric Focusing (IEF)
• 2-D Electrophoresis
Supporting Documentation
• Flamingo Fluorescent Gel Stain Product Information Sheet
• Flamingo Fluorescent Gel Stain Instruction Manual
• Imaging Fluorescently Stained Gels with Image Lab Software Quick Start Guide
Online Resources
Staining and Visualization of Proteins After 2-D Electrophoresis
