Bio-Rad, 1610771, 10x Tris/Glycine Buffer for Western Blots and Native Gels

Name: 10x Tris/Glycine Buffer for Western Blots and Native Gels
Brand: Bio-Rad
SKU: 1610771

Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water, 10x Tris/Glycine Buffer for Western Blots and Native Gels

Catalog Number: 1610771

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Category

Blocking Reagents

TagsBuffers Reagents LSG PQB - Protein Quantitation Research Products Life Science Products Electrophoresis & Blotting Protein Electrophoresis and Blotting Equipment Electrophoresis>Electrophoresis Buffers and Reagents and Acrylamide for Protein Electrophoresis Gel Running Consumables Protein Running/Sample Buffer Running Buffers and Reagents

Description

Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.

• For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol

• For tank blotting of native gels, without methanol

• As a running buffer for native gels

Precast gels that can be used for native electrophoresis

Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.

Packaging Options

Related Products

• PrecisionAb™ Validated Western Blotting Antibodies

• Native Sample Buffer ( 1610738 )

• Criterion Staining/Blotting Trays with lids ( 3459921 )

• 10x Phosphate Buffered Saline (PBS) ( 1610780 )

• 10x Tris Buffered Saline (TBS) (1706435)

• 1x Phosphate Buffered Saline (PBS) with 1% Casein ( 1610783 )

• 1x Tris Buffered Saline (TBS) with 1% Casein ( 1610782 )

• Blotting-Grade Blocker, nonfat dry milk ( 1706404 )

Related Categories

• Protein Gels

• Western Blotting Membranes and Filter Paper

• Semi-Dry and Rapid Blotting Systems

• Wet/Tank Blotting Systems

• Power Supplies

• Transfer Buffers

• Immunodetection Reagents and Kits

• Gel and Western Imaging Systems

Supporting Documents

• Mini-PROTEAN Precast Gels Instruction Manual and Application Guide

• Criterion Gel Application Guide Instruction Manual

• Mini Trans-Blot ® Cell Instruction Manual

• Trans-Blot SD Cell Quick Reference Guide

• Trans-Blot SD Semi-Dry Transfer Cell Instruction Manual

Online Applications

• Protein Electrophoresis

• Western Blotting

• Imaging and Analysis

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