Description
Use 10x Tris/CAPS Buffer for western transfer when using a semi-dry blotting apparatus. Prior to use, methanol needs to be added to a final concentration of 15% to the anode buffer, and SDS needs to be added to a final concentration of 0.1% to the cathode buffer.
• Discontinuous buffer system increases transfer efficiency
• Transfers take place in as little as 15–60 min
• Economical, low buffer requirements
Related Products
• Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell ( 1703940 )
• PrecisionAb Validated Western Blotting Antibodies
• Criterion Staining/Blotting Trays with lids ( 3459921 )
• 10x Phosphate Buffered Saline (PBS) ( 1610780 )
• 10x Tris Buffered Saline (TBS) ( 1706435 )
• 1x Phosphate Buffered Saline (PBS) with 1% Casein ( 1610783 )
• 1x Tris Buffered Saline (TBS) with 1% Casein ( 1610782 )
• Blotting-Grade Blocker, nonfat dry milk ( 1706404 )
Related Categories
• Protein Gels
• Western Blotting Membranes and Filter Paper
• Semi-Dry and Rapid Blotting Systems
• Power Supplies
• Transfer Buffers
• Immunodetection Reagents and Kits
• Gel and Western Imaging Systems
Supporting Documents
• Trans-Blot SD Cell Quick Reference Guide
• Trans-Blot SD Semi-Dry Transfer Cell Instruction Manual
Online Applications
• Western Blotting
• Protein Electrophoresis
