Description
Use ProteoMiner Protein Enrichment Large-Capacity Kits to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins in ≥50 mg of sample. The protein complexity of the sample is maintained.
The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away.
• Knowledge of the identity and characteristics of the proteins within a sample is not required
• Compatible with a wide range of sample types
• Narrows the range of protein concentrations within a sample
• Can be used for differential expression analysis
• Compatible with most downstream applications including 1-D and 2-D electrophoresis and mass spectrometry
For fractionation of the protein sample after binding to the beads, the ProteoMiner Protein Enrichment Small-Capacity Kit can be used with the ProteoMiner Sequential Elution Reagents (1633003). The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics. The ProteoMiner Protein Enrichment Large-Capacity Kit and Sequential Elution Reagents can be purchased together in one kit, the ProteoMiner Sequential Elution Large-Capacity Kit (1633011). Sequential elution has been optimized for samples for SELDI. Elution reagents are not compatible with direct use of fractions in 2-D gel electrophoresis.
Kit Contents
• Spin columns, 10; 500 µl bead slurry (20% beads, 20% v/v EtOH), 100 µl settled bead volume
• Wash Buffer, 150 mM NaCl, 10 mM NaH 2 PO 4 , pH 7.4, 50 ml
• Elution Reagent, lyophilized 8 M urea, 2% CHAPS, 5 vials
• Rehydration Reagent, 5% acetic acid, 5 ml
• Capless 2 ml centrifuge tubes, 20
• Capped 2 ml centrifuge tubes, 10
• Instruction Manual
• Quick Start Guide
Product Options
Related Products
• Mini Bio-Spin ® Chromatography Columns ( 7326207 )
• ReadyPrep™ Proteomics Grade Water ( 1632091 )
• ReadyPrep 2-D Cleanup Kit
Related Categories
• Centrifuges
• Protein Electrophoresis and Blotting
• 2-D Electrophoresis
• Protein Assay Kits and Cuvettes
• Protein Fractionation
• Magnetic Beads and Racks for Immunoprecipitation
Supporting Documentation
• ProteoMiner Protein Enrichment and Sequential Kit Protocols
• ProteoMiner Sequential Elution Kit Protocol
• ProteoMiner Protein Enrichment Kits Instruction Manual
• ProteoMiner Protein Enrichment Technology Brochure
• Enrichment of Medium- and Low-Abundance Proteins in Sample Types Using ProteoMiner Technology Application Note
