Bio-Rad, 17007153EDU, Fish DNA Barcoding Kit with Agarose and TAE

Name: Fish DNA Barcoding Kit with Agarose and TAE
Brand: Bio-Rad
SKU: 17007153EDU

PCR and bioinformatics kit bundle, includes Fish DNA Barcoding Kit (17007432EDU) and reagents for agarose gel electrophoresis of up to 16 fish DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation); for educational use only, Fish DNA Barcoding Kit with Agarose and TAE

Catalog Number: 17007153EDU

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Category

Agarose

TagsLSG EDU - Bio-Education Science Education Classroom Educational Kits Kits Amplification>EDU_Kits DNA Barcoding Kits PCR and Real-Time PCR Kits

Description

Teach PCR, gel electrophoresis, and bioinformatics with this DNA barcoding lab activity in which students determine the species of a fish based on the DNA sequence of its cytochrome c oxidase I (COI) gene. Students first extract DNA from tissue, use PCR to amplify the DNA, and perform agarose gel electrophoresis to verify PCR products. Next, after receiving DNA sequencing results from a third party (sequencing services not included), students then use bioinformatics to genetically identify the species using the sequence obtained. The unique COI sequence produced for a given sample is considered its DNA barcode.

This cost-saving bundle includes agarose and TAE electrophoresis buffer.

With this kit, you can teach your students how to:

• Perform DNA extraction, PCR amplification, and gel electrophoresis up to 16 fish DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation)

• Use DNA sequencing and bioinformatics to determine species identity

• Perform investigations in their area to examine biodiversity or see if marketplace substitution is occurring

• Contribute to global initiatives for barcoding life (Barcode of Life Database)

Learn more on the DNA Barcoding Kits product page.

Timeline

Finish all lab activities in three 45-minute class periods.

Product Contents

• DNA Extraction Module: reagents and consumable plastics for DNA extraction ( 12016408EDU )

• Fish PCR Module: reagents and consumable plastics for PCR amplification of the fish COI gene, control DNA, DNA loading dye and stain, and molecular weight ruler ( 12016300EDU )

• Certified molecular biology agarose, 25 g ( 1613100EDU )

• 50x Tris/Acetic Acid/EDTA (TAE), 100 ml ( 1660742EDU )

Related Products

• Fish DNA Barcoding Kit ( 17007432EDU )

• Mammals, Insects, and Fungi DNA Barcoding Kit ( 17007366EDU )

• Mammals, Insects, and Fungi DNA Barcoding Kit with Agarose and TAE ( 17007135EDU )

• Fish, Mammals, Insect, and Fungi DNA Barcoding Kit ( 17007154EDU )

• Fish, Mammals, Insect, and Fungi DNA Barcoding Kit with Agarose and TAE ( 17007155EDU )

Ordering

Notice

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