Description
Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.
Key Features and Benefits
• Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
• Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1 pg total input RNA
• Amplify cDNA for genes with low expression levels
• Fast 26-minute 1-tube cDNA synthesis protocol
• 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination
Packaging Options
See the iScript Reverse Transcription Supermix product page for more information and documentation.
Related Products
• cDNA Synthesis Kits
• Real-Time PCR Supermixes and Kits
• PrimePCR™ PCR Primers & Assays
Related Categories
• PCR and qPCR Reagents
• Real-Time PCR Detection Systems
• PCR Plastic Consumables
• Instrument Validation Tools and Services
Supporting Documents
• iScript Reverse Transcription Supermix Product Insert
• Amplification Plastics and Reagents Selection Guide
Online Resources
• qPCR/Real-Time PCR
• Genomics
