Bio-Rad, 1708898, iScript™ RT-qPCR Sample Preparation Reagent

Packaging Options

• Catalog #170-8898 , 100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers

• Catalog # 170-8899 , 500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers

Description

iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.

Key Features and Benefits

• Have ease-of-use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5–10 min

• Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents

• Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells

• Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen

• Use with different cell types — compatible with both suspension and adherent cell cultures