Description
Use the Bio-Plex Pro RBM apoptosis panel 2 multiplex assay for detecting the intracellular proteins involved in the commitment, onset, and induction of apoptosis by the intrinsic pathway listed below:
• Bad
• Bax/Bcl-2 dimer
• Bcl-xL
• Bim
• Mcl-1
For use with the following sample types:
• Cell lysates
• Tissue lysates
Benefits
• Optimized for high precision and lot-to-lot reproducibility of sample measurements
• Magnetic beads for simplified plate processing
• 2-level quality controls with kit lot-specific ranges
• Assay quick guide to get you started right away
Kit Components
• 6.5 µm magnetic capture beads Detection antibodies Standards 2-level controls Standard diluent Blocking buffer Lysate dilution buffer (LDB)
• Cytosolic extraction buffer (CEB) 10x assay buffer 10x streptavidin-PE Flat bottom plate Plate seals Instructions
Other Available Apoptosis Assays
Run all three Bio-Plex Pro RBM apoptosis panels for a more complete picture of the intrinsic pathway:
• Panel 1 ( 171-WAR1CK ) detects Bak, Bax, Lamin B, and Smac
• Panel 2 (171-WAR2CK) detects Bad, Bax/Bcl-2 dimer, Bcl-xL, Bim, and Mcl-1
• Panel 3 ( 171-WAR3CK ) detects active caspase-3, Bcl-xL/Bak dimer, Mcl-1/Bak dimer, and survivin
Instrument Compatibility
Compatible with these readers and wash stations:
• Bio-Plex 200 or Luminex 200 system
• Bio-Plex 3D or Luminex FLEXMAP 3D system
• Bio-Plex ® MAGPIX™ or Luminex MAGPIX system
• Bio-Plex Pro™ wash stations
More Information
To learn more about performance characteristics and other cancer-related panels, refer to the documents listed below:
• Product information sheet
• Instruction manual
• Quick guide
• Cancer panels product information sheet
• Cell signaling product information sheet
