Description
Use the SingleShot Probes Kit to obtain high-quality RT-qPCR data with probe chemistry directly from cell culture lysates.
Two-step workflow provides results in less than 2 hours after cell lysis. All RT-qPCR reagents and RNA control included.
Benefits include:
• Cell lysate from 10 to 100,000 cells ready in 20 minutes
• Complete removal of genomic DNA while preserving RNA integrity
• Optimal accuracy and linear dynamic range of qPCR data across genes
• Results comparable to using purified RNA, including dynamic range
• Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )
• SingleShot RNA Control ensures optimal cell and lysate inputs
Optimized Sample Input
The SingleShot Probes Kit maximizes cell lysate and cDNA inputs in a two-step RT-qPCR reaction:
Kit Contents
Refer to the user manual for details on reaction setup and troubleshooting tips.
Cell Lines Validated for Use with the SingleShot Probes Kit
The SingleShot Probes Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:
Related Products
The SingleShot Probes Kit belongs to the family of SingleShot™ Cell Lysis RT-qPCR Kits:
• SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR ® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )
• SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR ® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )
• SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )
