Bio-Rad, 1725270, SsoAdvanced Universal SYBR<sup>®</sup> Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

Name: SsoAdvanced Universal SYBR ® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
Brand: Bio-Rad
SKU: 1725270

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl 2, SYBR ® Green I, ROX normalization dyes, for 200 x 20 µl reactions, SsoAdvanced Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)

Catalog Number: 1725270

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Category

Blocking Reagents

Pack Size200 x 20 µL

TagsReagents Research Products Life Science Products LSG GXB - Gene Expression Amplification>Real-time PCR Reagents PCR & qPCR PCR Reagents & qPCR Reagents Real-Time PCR Supermixes and Kits Supermix SYBR SsoAdv SsoAdvanced™ Universal Supermixes 200 x 20 µl rxns

Description

SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

SsoAdvanced Universal SYBR Green Supermix lets you:

• Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system

• Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges

• Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance

• Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation

• Obtain better results with Bio-Rad's PrimePCR assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance

Instrument Compatibility

SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal SYBR Green Supermix

• qPCR/real-time PCR

• Gene expression analysis

• Pathway analysis

• Absolute quantification

• Chromatin immunoprecipitation (ChIP) qPCR

• Mutation detection

• Pathogen detection

• Viral detection (load)

• Characterization of genetically modified organisms (GMO)

• Genetic profiling

Packaging Options

Related Products

We have developed SsoAdvanced universal probes supermix, which is available in several formats (200–5,000 reactions).

Bio-Rad offers a complete workflow solution for real-time PCR, including:

• RNA isolation kits

• Reverse transcription kits

• Real-time PCR supermixes

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patent 7,560,260.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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