Bio-Rad, AHP3064, Di-Methyl-Histone H3 (Lys79) antibody

Rabbit anti Human di-methyl-histone H3 (Lys79) antibody recognizes histone H3 when di-methylated at lysine 79. Histone H3 is one of the four core histones that make up the nucleosome core particle. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) ( Smith, 1991 ). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression ( Gupta et al . 2010 ). Lysine 79°F histone H3 may be mono-, di- or tri-methylated by the methylase DOT1. Methylation of lysine 79°F histone H3 acts as a marker of inactive chromatin regions that is essential for silencing of transcription of proteins such as SIR3 ( Ng et al . 2003 ). Wide species cross-reactivity is expected from Rabbit anti Human di-methyl-histone H3 (Lys79) antibody based on sequence.

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.