Bio-Rad, AHP3071, Histone H4 (Ac12) antibody

Rabbit anti Human histone H4 (Ac12) antibody recognizes histone H4 when acetylated at lysine 12. Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) ( Smith, 1991 ). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation ( Koch et al . 2007 ). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions ( Kuo and Allis, 1998 ). Acetylation of histone H4 usually results in transcriptional activation ( Turner et al . 1989 ). Wide species cross-reactivity is expected from Rabbit anti Human histone H4 (Ac12) antibody based on sequence.

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.