Bio-Rad, BUF012A, alamarBlue®
Size: 25 ml, alamarBlue®
Catalog Number: BUF012A
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Category
Pack Size25 mL
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| Product Form | BUF012B: Liquid. |
|---|---|
| Preservative Stabilisers | BUF012B: None present. |
| Regulatory | BUF012B: For research purposes only. |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| Product Type | Accessory Reagent |
| Specificity | alamarBlue® |
| Intended Use | BUF012B •Cell proliferation assays. • The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay. •Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo. toxicity. •alamarBlue ® is for use between pH6.8 and pH7.4. |
| Test Principle | BUF012B Cell proliferation assay •Growing cells cause a chemical reduction of alamarBlue. •Continued growth maintains a reduced environment. (fluorescent, red). •Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue). • Data may be collected using either fluorescence-based or absorbance-based instrumentation. •Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength. •Absorbance is monitored at 570nm and 600nm. |
| Acknowledgements | Manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959. |
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