Bio-Rad, LNK002P, HRP Conjugation Kit
Size: 3 Conjugations For 400µg Antibody, LYNX Rapid HRP Antibody Conjugation Kit
Catalog Number: LNK002P
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Category
Pack Size3 vials
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| Regulatory | For research purposes only |
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| Guarantee | 12 months from date of despatch |
| Product Type | Conjugation Kit |
| Specificity | HRP Conjugation Kit |
| Reagents In The Kit | LNK006P: 3 Vials of 10μg LYNX lyophilized HRP mix 1 Vial LYNX Modifier reagent 1 Vial LYNX Quencher reagent |
| Preparing The Antibody | LNK006P The following buffer solutions are recommended for preparing the antibody: 10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated. If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals . EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency. Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided. The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 10μg HRP you need to add between 10-40μg of antibody. For optimal results the antibody volume should be up to 10μl, at a concentration range of 0.5-5.0mg/ml. |
Answer:The kit provides three HRP conjugations.
Answer:Each conjugation is designed for up to approximately 400 µg of antibody.
Answer:The kit includes three lyophilized HRP-mix vials, Modifier reagent, and Quencher reagent.
LYNX Rapid HRP Antibody Conjugation Kit enables covalent labeling of antibodies or other compatible proteins with horseradish peroxidase. This configuration provides three conjugations for up to 400 µg antibody per conjugation. The kit contains three vials of 100 µg lyophilized HRP mix, one vial of Modifier reagent, and one vial of Quencher reagent. Conjugation is performed near neutral pH using an amine-free buffer, with an incubation of approximately three hours at room temperature or overnight if preferred. The directional chemistry is designed for high conjugation efficiency and recovery without a separate purification step, supporting preparation of HRP-labeled antibodies for ELISA, immunoassays, and related detection workflows.
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| Licensed Use |
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| These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting Bio-Rad, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail: [email protected] |
