Bio-Rad, MCA5862G, Alpha Fetoprotein antibody | 125.6G7

Mouse anti Human alpha fetoprotein, clone 125.6G7 is a monoclonal antibody recognizing alpha fetoprotein (AFP), a member of the albuminoid protein superfamily, along with albumin and vitamin D binding protein. Members of the albuminoid superfamily comprise molecules with three characteristic globular domains ( Mizejewski 1995 ) . AFP is a ~70 kDa plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. AFP acts as a transport protein for various ligands such as bilirubin, fatty acids, steroids and heavy metals. After birth, plasma AFP levels drop rapidly with only trace amounts remaining in healthy adults. Changes in plasma levels of AFP have been documented in a variety of cancers including hepatocellular cancer ( Sato et al. 1993 ) Clone 125.6G7 shows <0.1% reacativity with human serum albumin and IgG. Strong expression of AFP has been noted in the hepatic cell line
HepG2 .

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.