Description
Human Anti-Belimumab Antibody, clone AbD59897 is a non-inhibitory, anti-idiotypic antibody that specifically recognizes free human monoclonal antibody drug belimumab, and the drug in complex with its target, the B-cell activating factor BAFF (CD257). The antibody does not recognize free BAFF and does not inhibit the binding of belimumab to BAFF. AbD59897 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA0102 . It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 ( TZC002P ) for use in direct ELISA under product code TZA0102P . A pair of anti-belimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay. Clone AbD59900ad ( TZA0101 ) is recommended as the capture antibody paired with clone AbD59897 as the detection antibody, either in purified ( TZA0102 ) or HRP conjugated ( TZA0102P ) format. Belimumab (trade name Benlysta) is a fully human monoclonal antibody of the IgG1/lambda isotype that binds to the B-cell activating factor BAFF to prevent its binding to B cells. Although part of the normal immune system, B cells are also responsible for the over-aggressive immune response observed in autoimmune diseases, such as systemic lupus erythematosus (SLE). Through its binding to BAFF, belimumab makes BAFF unavailable to B cells, which induces B cells apoptosis and therefore, a reduction in the overall number of circulating B cells. Therefore, belimumab acts as an immunosuppressant drug and is approved for the treatment of systemic lupus erythematosus. View a summary of all anti-belimumab antibodies
Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
