Biolegend, 110717, Alexa Fluor® 488 anti-mouse CD45.1 Antibody
25microg
Catalog Number: 110717
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | SJL mouse thymocytes and splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)). |
| Application References(PubMed link indicates BioLegend citation) | Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed |
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| Product Citations | Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed Alam J, et al. 2022. Front Med (Lausanne). 9:849990. PubMed Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed Fumagalli V, et al. 2020. J Exp Med. :217. PubMed El Amki M, et al. 2020. Cell Rep. 33:108260. PubMed Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed Sharma S, et al. 2015. J Immunol. 194:5529. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed Alam J, et al. 2021. Front Immunol. 12:701415. PubMed Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed Wu T, et al. 2014. J Leukoc Biol. 95:215. PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed Tang Q, et al. 2021. Immunity. 54:1961. PubMed Hirasawa M, et al. 2016. J Biol Chem. 291: 7373-7385. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Allman W, et al. 2015. Proc Natl Acad Sci U S A. 112: 4094 - 4103. PubMed |
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| RRID | AB_492863 (BioLegend Cat. No. 110717) AB_492862 (BioLegend Cat. No. 110718) |
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| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
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| Distribution | All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice |
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| Function | Phosphatase, T and B cell activation |
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| Ligand/Receptor | Galectin-1, CD2, CD3, CD4 |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
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| Gene ID | 19264 |
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| UniProt | View information about CD45.1 on UniProt.org |
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| Clone | A20 |
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| Regulatory Status | RUO |
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| Other Names | T200, Ly-5.1, LCA |
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| Isotype | Mouse (A.SW) IgG2a, κ |
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