Biolegend, 358610, Brilliant Violet 421™ anti-human LOX-1 Antibody
100tests
Catalog Number: 358610
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | LOX-1 ectodomain: Fc fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
Answer:Brilliant Violet 421™ Anti-Human LOX-1 Antibody, 358610.
Answer:Human LOX-1; BV421; Flow cytometry.
Answer:Brilliant Violet 421™ anti-human LOX-1 recognizes the lectin-like oxidized low-density lipoprotein receptor encoded by OLR1.
Brilliant Violet 421™ anti-human LOX-1 recognizes the lectin-like oxidized low-density lipoprotein receptor encoded by OLR1. LOX-1 is studied in lipid handling, vascular inflammation, and the phenotype of certain myeloid cell populations. The antibody provides violet-laser-excited fluorescence for analysis of LOX-1 expression by flow cytometry using compatible staining and detection settings. It can be combined with other cell-surface markers to investigate LOX-1-positive populations in experimental immune and vascular biology studies. The reagent is intended for research use. Selection of controls, titration, and interpretation should reflect the tested sample type; no clone or package quantity is inferred from the broader antibody product line.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunofluorescent staining of acetone-fixed frozen human skin sections1. |
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| Application References(PubMed link indicates BioLegend citation) | Li D, et al. 2012. J. Exp. Med. 209:109. (IHC) |
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| Product Citations | Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed Koh J, et al. 2020. Eur J Immunol. 50:1810. PubMed |
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| RRID | AB_2728342 (BioLegend Cat. No. 358609) AB_2728343 (BioLegend Cat. No. 358610) |
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| Structure | Single pass, type II, transmembrane protein, 31 kD, homodimer, member of the C-type lectin superfamily. |
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| Distribution | Endothelial cells, smooth muscle, platelets, fibroblasts, macrophages; Upregulated by inflammatory and oxidative stimuli |
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| Function | Scavenger receptor, involved in endocytosis, phagocytosis and cytokine production |
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| Ligand/Receptor | Recognizes numerous ligands including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, oxidized LDL |
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| Cell Type | Endothelial cells, Platelets, Fibroblasts, Macrophages, Dendritic cells |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules |
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| Antigen References | 1. Yin Y, et al. 2013. Eur. J. Cell Biol. 92:150. 2. Khaidakov M. and Mehta JL. 2012. PLoS One. 7:e46973. 3. Sawamura T, et al. 2012. Curr. Opin. Lipidol. 23:439. 4. Lin FY, et al. 2011. J. Immunol. 186:4405. 5. Parlato S, et al. 2010. Blood 115:1554. |
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| Gene ID | 4973 |
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| UniProt | View information about LOX-1 on UniProt.org |
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| Clone | 15C4 |
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| Regulatory Status | RUO |
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| Other Names | OLR1, LOX1, LOXIN, SLOX1, CLEC8A, SCARE1 |
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| Isotype | Mouse IgG2a, κ |
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