Biolegend, 300406, FITC anti-human CD3 Antibody

Name: FITC anti-human CD3 Antibody
Brand: Biolegend
SKU: 300406

100tests

Catalog Number: 300406

Pricing

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General

Verified Reactivity	Human
Reported Reactivity	Chimpanzee
Antibody Type	Monoclonal
Host Species	Mouse
Formulation	µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation	The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration	µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application	FC - Quality tested
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

FAQs

Question: How does the 100-test size differ from the larger format?

Answer:The antibody, clone and FITC conjugate are the same; the 100-test pack supplies fewer staining tests than the 500-test format.

Question: Which cell population does FITC anti-human CD3 identify?

Answer:It labels CD3-expressing human T cells for flow-cytometry immunophenotyping.

Question: What are the storage conditions?

Answer:Keep the antibody at 2–8°C, protect it from light and do not freeze.

Application Note

BioLegend FITC Anti-Human CD3 is a fluorochrome-conjugated mouse monoclonal antibody for detecting the CD3ε subunit of the human T-cell receptor complex. The UCHT1 clone is used to identify mature T cells and related CD3-expressing populations in single-color or multicolor flow-cytometric immunophenotyping. This 100-test presentation is convenient for smaller studies, initial panel setup and routine quality-control staining without purchasing the larger 500-test pack. FITC fluorescence is excited by a 488 nm blue laser and is detected in an appropriate green channel. The test-size reagent contains BSA and sodium azide. A staining volume of 5 µL per million cells in 100 µL or per 100 µL whole blood provides a starting point, followed by titration for the cell type and instrument. Include relevant compensation and staining controls when other fluorochromes are present. Store the antibody at 2–8°C away from light and do not freeze.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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