Biolegend, 300440, FITC anti-human CD3 Antibody

Name: FITC anti-human CD3 Antibody
Brand: Biolegend
SKU: 300440

500tests

Catalog Number: 300440

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General

Verified Reactivity	Human
Reported Reactivity	Chimpanzee
Antibody Type	Monoclonal
Host Species	Mouse
Formulation	µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation	The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration	µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application	FC - Quality tested
Recommended Usage	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

FAQs

Question: How many flow-cytometry tests are supplied?

Answer:This package contains 500 tests of FITC-conjugated anti-human CD3.

Question: Which clone and fluorochrome are used?

Answer:The monoclonal antibody uses clone UCHT1 with FITC labeling, excited by a 488 nm blue laser.

Question: Is this FITC antibody suitable for T-cell activation experiments?

Answer:It is optimized as a flow-cytometry staining reagent and contains sodium azide; select an appropriate azide-free functional antibody for activation assays.

Application Note

BioLegend FITC Anti-Human CD3 is a mouse monoclonal antibody that identifies human CD3-positive T lymphocytes by flow cytometry. Its UCHT1 clone recognizes the CD3ε component of the T-cell receptor complex, and the FITC fluorochrome is excited by a 488 nm blue laser. The 500-test size suits repeated T-cell phenotyping, high-throughput sample panels and workflows requiring the same immunophenotyping marker across many specimens. For standard test-size staining, 5 µL can be used per million cells in 100 µL staining volume or per 100 µL of whole blood; titrate when sample characteristics require adjustment. Use suitable negative controls and compensation to distinguish FITC signal from other fluorochromes. The antibody is supplied in a buffered solution containing sodium azide and BSA, making it a research staining reagent rather than an azide-free functional antibody preparation. Store at 2–8°C, protected from light, without freezing.

Ordering

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