Biolegend, 423102, Zombie Aqua™ Fixable Viability Kit

The BioLegend Zombie Aqua™ Fixable Viability Kit is an amine-reactive fluorescent staining system for discriminating live cells from cells with compromised membranes in flow cytometry. Healthy cells largely exclude the dye, while membrane-damaged cells take up more reagent and develop a stronger fluorescent signal following reaction with cellular amines. The 500-test package contains five vials of lyophilized Zombie Aqua dye with anhydrous DMSO for reconstitution. The dye is excited by a 405 nm violet laser and emits near 516 nm, so the panel must account for overlap with BV510-like fluorochromes. Perform the viability staining step in protein-free PBS and avoid Tris, serum and BSA until staining is complete, because free amines interfere with labeling. The covalent dye reaction allows stained samples to be fixed for subsequent analysis. Store unopened kit components at −20°C and protect reconstituted reagent from moisture and light.