Biolegend, 110727, PerCP/Cyanine5.5 anti-mouse CD45.1 Antibody
25microg
Catalog Number: 110727
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | SJL mouse thymocytes and splenocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)). |
| Application References(PubMed link indicates BioLegend citation) | Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed |
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| Product Citations | Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed Melzi E, et al. 2022. Immunity. 55:2168. PubMed Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed Wang Y, et al. 2022. Cell Rep. 41:111738. PubMed Pleuger C, et al. 2022. Elife. 11: . PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Li X, et al. 2022. Cell. 185:1709. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Liang Y, et al. 2020. J Immunol. 204:2984. PubMed Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Inoue D, et al. 2021. Nat Genet. 53:707. PubMed Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed Marangoni F, et al. 2021. Cell. . PubMed Yoshimi A, et al. 2019. Nature. 574:273. PubMed Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed Kim C, et al. 2019. Cell Rep. 29:2202. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed Quah B, et al. 2012. Cytometry A. 81:679. PubMed Karimi M, et al. 2015. Blood. 125:3655. PubMed Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed Krueger CC, et al. 2019. Front Immunol. 10:1831. PubMed Sharma S, et al. 2021. Pathophysiology. 28:501. PubMed Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Ma K, et al. 2022. iScience. 25:104347. PubMed Wang X, et al. 2021. EMBO J. 40:e105926. PubMed Yuan X, et al. 2017. Elife. 6:e29540. PubMed Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed Lamb T, et al. 2022. Toxics. 10:. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed Ehrens A, et al. 2021. Cell Reports. 34(2):108621. PubMed |
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| RRID | AB_893348 (BioLegend Cat. No. 110727) AB_893346 (BioLegend Cat. No. 110728) |
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| Structure | Protein tyrosine phosphatase (PTP) family, 180-240 kD |
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| Distribution | All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice |
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| Function | Phosphatase, T and B cell activation |
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| Ligand/Receptor | Galectin-1, CD2, CD3, CD4 |
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| Biology Area | Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851. |
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| Gene ID | 19264 |
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| UniProt | View information about CD45.1 on UniProt.org |
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| Clone | A20 |
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| Regulatory Status | RUO |
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| Other Names | T200, Ly-5.1, LCA |
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| Isotype | Mouse (A.SW) IgG2a, κ |
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