Biolegend, 317421, Alexa Fluor® 647 anti-human CD4 Antibody
25tests
Catalog Number: 317421
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human peripheral T cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified |
| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9 |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81. |
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| Product Citations | Izquierdo-Useros N, et al. 2012. PLoS Biol. 10:e1001448. PubMed Greenwood EJD et al. 2019. Cell Rep. 27(5):1579-1596 . PubMed Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed Veazey R, et al. 2016. Mucosal Immunol. 10.1038/mi.2015. PubMed Carter JA, et al. 2020. Cell Systems. 9(5):475-482.e4.. PubMed |
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| RRID | AB_571941 (BioLegend Cat. No. 317422) |
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| Structure | Ig superfamily, type I transmembrane glycoprotein, 55 kD |
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| Distribution | T cell subset, majority of thymocytes, monocytes/macrophages |
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| Function | MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor |
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| Ligand/Receptor | MHC class II molecules, HIV gp120, IL-16 |
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| Cell Type | Macrophages, Monocytes, T cells, Thymocytes, Tregs |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723. |
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| Gene ID | 920 |
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| UniProt | View information about CD4 on UniProt.org |
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| Clone | OKT4 |
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| Regulatory Status | RUO |
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| Workshop | HCDM listed |
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| Other Names | T4 |
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| Isotype | Mouse IgG2b, κ |
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