Biolegend, 425205, Calcein Red-AM
10x25microg
Catalog Number: 425205
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Category
Pack Size10 vials
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| Formulation | Lyophilized |
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| Preparation | Calcein Red-AM labeling kit consists of lyophilized Calcein Red-AM and anhydrous DMSO. For reconstitution, bring the kit to room temperature and add 25 µL of DMSO to one vial of Calcein Red-AM dye until fully dissolved. |
| Storage & Handling | Calcein Red-AM should be stored at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Calcein Red-AM, use immediately or store at -20°C in a dry place and protected from light, preferably in a desiccator or in a container with desiccant for no more than a month. |
| Application | FC - Quality testedLive cell imaging - Verified |
| Recommended Usage | This lot has been tested by flow cytometric analysis of cell vitality. It can be used at concentrations ranging from 0.025 – 0.006 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application. |
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| Application Notes |
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| The molecular weight of Calcein Red-AM is 1015.79 Da. The maximum excitation and emission wavelengths of Calcein Red-AM are 560 nm/574 nm, respectively. Each 25 µg of Calcein-AM may be reconstituted with 25 µL of anhydrous DMSO to yield a stock concentration of 1 mM.Materials Provided:10 vials x 25 µg Calcein Red-AM1 vial of 500 µL anhydrous DMSOCalcein-AM Labeling Procedure:1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.2. Prepare a 1 mM stock solution by reconstituting 1 vial of lyophilized Calcein Red-AM dye with 25 µL of anhydrous DMSO.3. Prepare a 1 µM working solution by diluting 1 µL of 1 mM Calcein Red-AM stock solution in 1 mL PBS.4. Spin down cells and adjust the cell suspension to 1x107 cells/mL in PBS.5. Add 6 µL of the 1 µM Calcein Red-AM working solution to each mL of cell suspension for a final concentration of 0.006 µM.6. Incubate cells for 30 minutes at room temperature or at 37°C and keep protected from light.7. Pellet cells and resuspend in pre-warmed cell culture medium.8. Incubate cells for 10 minutes to ensure optimal retention of the Calcein Red-AM.9. After incubation, Calcein Red-AM labeled cells are ready for downstream applications or analysis. |
| Structure | Acetoxymethyl ester |
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| Distribution | Cytoplasmic |
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| Function | Cell vitality and health probe. Shows the pathway to death in more detail. |
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| Interaction | Nonspecific esterases |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Calcein, Calcien Red-AM |
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